The yulink was expressed in whole zebrafish embryo ubiquitously from 0.5 hpf (zygote stage) to 3 dpf (larval stage) with lateral overview. To address this question, we established an ex vivo system to culture zebrafish blastoderm tissue explants lacking the yolk cell and its associated YSL. I could find a few publications using EdU staining in zebrafish embryos, and referring to the Invitrogen Kit. Phox2a is first detected in LC … After hybridization with Dig-labeled antisense or control sense RNA probes of yulink, embryos were incubated with anti-Dig antibody conjugated to AP and developed with NBT-BCIP reagents. 1 This transparent vertebrate system develops rapidly, and already after 48 h a beating heart and robust blood circulation are readily observable under a stereomicroscope. We report that the zebrafish mutation soulless, in which the development of locus coeruleus (LC) noradrenergic (NA) neurons failed to occur, disrupts the homeodomain protein Phox2a. • The investigator should identify the location of the incubators on the Animal Study Proposal. • Incubators containing zebrafish embryos should be labeled as ‘Contains Live Zebrafish Embryos’. In the last 20 years, the zebrafish has proven to be an excellent model organism to study neurogenesis in the embryo. The reduced versions, Red CM-H 2-XRos and Orange CM-H 2-TMRos, are preferred since they are non-fluorescent until entering … • The zebrafish incubator should be labeled “Zebrafish Incubator” so it is easily identifiable during ACUC inspections. The shield is an accumulation and condensation of DEL cells along a local stretch of about 30 degrees of longitude of the germ ring, and marks the future dorsal side of the embryo. Zebrafish have transparent embryos that develop outside the mother which is an important feature for microscopy. Zebrafish Neuromast Labeling Protocols 12/2/05 pattern that allows ready distinction from neuromast cells. For this, blastoderm explants were prepared from embryos at early cleavage stages (256 cell stage), before YSL formation or germ layer induction, and kept in serum-free medium until stage-matched control embryos … Fig. The zebrafish embryo is rapidly becoming an attractive tool for screening nanoparticles. Zebrafish are vertebrates often used for in vivo studies, because they are relatively small in size and the embryonic and larval development is ex utero and fast, since within 5 days post-fertilization (dpf), all major organ systems are formed (Kimmel et al., 1995).Zebrafish embryos are virtually … They can be raised so that mutants can be readily screened and propagated. Fluorescently labelled 70 nm SiO2 nanoparticles were injected into the bloodstream of 3 days old transgenic zebrafish embryos and live … Zebrafish As a Model Organism for Developmental Disorders. External as well as internal cues orchestrate neurogenesis in a precise temporal and spatial way. Neurogenesis in the developing central nervous system consists of the induction and proliferation of neural progenitor cells and their subsequent differentiation into mature neurons. Formation of the embryonic shield visibly indicates that rapid convergence movements have begun. Phox2a is not only necessary but also sufficient to induce Phox2b+ dopamine-beta-hydroxylase+ and tyrosine hydroxylase+ NA neurons in ectopic locations. 5 (a) Representative 3D confocal fluorescence images of zebrafish embryos (at 72 hpf) after treatment with Au NPs (5 μg mL −1) or GO nanosheets (50 μg mL −1). 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